CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids.
نویسندگان
چکیده
We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.
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ورودعنوان ژورنال:
- Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
دوره 11 6 شماره
صفحات -
تاریخ انتشار 2012